Regulatory
PAtpI

Part:BBa_K1930005:Design

Designed by: Eike Mahlandt   Group: iGEM16_Groningen   (2016-10-16)


PAtpI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The promoter PAtpI is a constitutive promoter which has its origin in Bacillus subtilis. It is responsible for the expression of atpA gene (ATP synthesis) during the first 30 minutes of the germination of B. subtilis. atpA gene is part of an operon (atpI-atpB-atpE-atpF-atpH-atpA-atpG-atpD-atpC), therefore the promoter region in front of the first protein coding gene (atpI) in this operon was chosen.

For more information see plasmid construction under our lab journal: (BBa_K1930005.)

Source

Ordered as gBlock. IDT (Integrated DNA technologies).

References

Sinai et al. 2015

Subtiwiki-atpA